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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-4-4
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pubmed:abstractText |
The cDNA coding for mouse fibroblast tropomyosin isoform 2 (TM2) was placed into a bacterial expression vector to produce a fusion protein containing glutathione-S-transferase (GST) and TM2 (GST/TM2). Glutathione-Sepharose beads bearing GST/TM2 were incubated with [35S]methionine-labeled NIH 3T3 cell extracts and the materials bound to the fusion proteins were analyzed to identify proteins that interact with TM2. A protein of 10 kD was found to bind to GST/TM2, but not to GST. The binding of the 10-kD protein to GST/TM2 was dependent on the presence of Ca2+ and inhibited by molar excess of free TM2 in a competition assay. The 10-kD protein-binding site was mapped to the region spanning residues 39-107 on TM2 by using several COOH-terminal and NH2-terminal truncation mutants of TM2. The 10-kD protein was isolated from an extract of NIH 3T3 cells transformed by v-Ha-ras by affinity chromatography on a GST/TM2 truncation mutant followed by SDS-PAGE and electroelution. Partial amino acid sequence analysis of the purified 10-kD protein, two-dimensional polyacrylamide gel analysis and a binding experiment revealed that the 10-kD protein was identical to a calcium-binding protein derived from mRNA named pEL98 or 18A2 that is homologous to S100 protein. Immunoblot analysis of the distribution of the 10-kD protein in Triton-soluble and -insoluble fractions of NIH 3T3 cells revealed that some of the 10-kD protein was associated with the Triton-insoluble cytoskeletal residue in a Ca(2+)-dependent manner. Furthermore, immunofluorescent staining of NIH 3T3 cells showed that some of the 10-kD protein colocalized with nonmuscle TMs in microfilament bundles. These results suggest that some of the pEL98 protein interacts with microfilament-associated nonmuscle TMs in NIH 3T3 cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-1370827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-1512251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-1769063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2061300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2071603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2139656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2150518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2229046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2317800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2380244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2400784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2550459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2649250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2954560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-2998764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3162911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3221870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3242591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3244365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3336357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3384851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3422491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3514600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3595858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3606745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3624279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3628004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3722149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3754329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3793760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-3888373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-4000123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-4055761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-4113792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6133866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6208481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6272310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6282338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6306001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6315714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6328499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6383715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6605200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6771253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6838847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6842592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6890875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-6934513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-7016589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-7250124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-7358670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-8421055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-8455951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8120097-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
124
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pubmed:geneSymbol |
v-Ha-ras
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
757-68
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pubmed:dateRevised |
2010-10-6
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pubmed:meshHeading |
|
