Linked Life Data

8118888

Source:http://linkedlifedata.com/resource/pubmed/id/8118888

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-4-6
pubmed:abstractText
Analysis of the relationship between the structure of a protein molecule and its function often exploits the techniques of gene mutation and expression in transfected cell lines. This approach has been used extensively in the study of MHC molecules for testing predictions derived from structural models. Comparison of the functional properties of mutant molecules is difficult because MHC molecules interact with both peptides and T cell receptors. Functionality is commonly determined in biological assays which are dependent on T cell recognition of specific peptide/MHC complexes and measure secondary events triggered by T cell activation. In this study four L cell lines transfected with different combinations of alpha and beta chains from I-Ak and I-Au were used as antigen-presenting cells to activate two hen egg-white lysozyme-specific T cell clones. We compared several biological assays, namely, T cell proliferation, cytokine production, and cytotoxicity. Different assays indicated varying degrees of functionality for the same MHC molecule and thus demonstrated the difficulty in unambiguous interpretation of data from complex assays. Furthermore, we detected differences among the transfected lines which appeared unrelated to the expression of the introduced MHC genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Complications in the functional analysis of transfected MHC genes.
pubmed:affiliation
Department of Microbiology, University of California at Los Angeles 90024.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.