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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1994-4-7
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pubmed:abstractText |
The tumor growth suppressor WAF1/CIP1 was recently shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinases. In the present studies, we sought to determine the relationship between the expression of WAF1/CIP1 and endogenous regulation of p53 function. WAF1/CIP1 protein was first localized to the nucleus of cells containing wild-type p53 and undergoing G1 arrest. WAF1/CIP1 was induced in wild-type p53-containing cells by exposure to DNA damaging agents, but not in mutant p53-containing cells. The induction of WAF1/CIP1 protein occurred in cells undergoing either p53-associated G1 arrest or apoptosis but not in cells induced to arrest in G1 or to undergo apoptosis through p53-independent mechanisms. DNA damage led to increased levels of WAF1/CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. These results support the idea that WAF1/CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
54
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pubmed:geneSymbol |
WAF1/CIP1,
p53,
v-myc
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
1169-74
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8118801-Alleles,
pubmed-meshheading:8118801-Animals,
pubmed-meshheading:8118801-Apoptosis,
pubmed-meshheading:8118801-Cell Nucleus,
pubmed-meshheading:8118801-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:8118801-Cyclins,
pubmed-meshheading:8118801-DNA Damage,
pubmed-meshheading:8118801-G1 Phase,
pubmed-meshheading:8118801-Gene Expression,
pubmed-meshheading:8118801-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8118801-Genes, p53,
pubmed-meshheading:8118801-Humans,
pubmed-meshheading:8118801-Lymphoma, T-Cell,
pubmed-meshheading:8118801-Mice,
pubmed-meshheading:8118801-Mutation,
pubmed-meshheading:8118801-Protein Kinase Inhibitors,
pubmed-meshheading:8118801-Protein Kinases,
pubmed-meshheading:8118801-Tumor Cells, Cultured,
pubmed-meshheading:8118801-Tumor Suppressor Protein p53
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pubmed:year |
1994
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pubmed:articleTitle |
WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis.
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pubmed:affiliation |
Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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