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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-4-4
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pubmed:databankReference |
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pubmed:abstractText |
We cloned and sequenced feline interleukin-6 (IL-6) cDNA using the polymerase chain reaction (PCR) technique to obtain information on regulation of the IL-6 gene. The feline IL-6 cDNA cloned in this study was 752 bp long and included all the amino acid coding region. At the amino acid level, the overall identities of the feline IL-6 to the human and murine counterparts were 58% and 39%, respectively. After transfection with the expression vector containing the cDNA, CRFK cells produced biologically active proteins that showed hybridoma growth promoting activity.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0916-7250
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
941-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8117820-Amino Acid Sequence,
pubmed-meshheading:8117820-Animals,
pubmed-meshheading:8117820-Base Sequence,
pubmed-meshheading:8117820-Cats,
pubmed-meshheading:8117820-Cell Line,
pubmed-meshheading:8117820-Cloning, Molecular,
pubmed-meshheading:8117820-DNA, Complementary,
pubmed-meshheading:8117820-DNA Primers,
pubmed-meshheading:8117820-Humans,
pubmed-meshheading:8117820-Interleukin-6,
pubmed-meshheading:8117820-Kinetics,
pubmed-meshheading:8117820-Mice,
pubmed-meshheading:8117820-Molecular Sequence Data,
pubmed-meshheading:8117820-Polymerase Chain Reaction,
pubmed-meshheading:8117820-Recombinant Proteins,
pubmed-meshheading:8117820-Restriction Mapping,
pubmed-meshheading:8117820-Sequence Homology, Amino Acid,
pubmed-meshheading:8117820-Transfection
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pubmed:year |
1993
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pubmed:articleTitle |
Molecular cloning of feline interleukin-6 cDNA.
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pubmed:affiliation |
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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