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pubmed-article:8117674pubmed:abstractTextThe purification and characterization of the peripheral antenna and the preliminary characterization of a carotenoid-protein complex from the purple-sulfur bacterium Chromatium purpuratum are described. The peripheral antenna of C. purpuratum is unusual among purple bacteria in that it can be resolved by SDS-PAGE into six subunits, the largest number observed thus far for a spectrally pure antenna complex. N-terminal sequence analyses of these subunits suggest that they may have an additional bacteriochlorophyll-binding site located outside the transmembrane domain. The results of pigment-protein quantification are also consistent with additional pigment-binding sites in the C. purpuratum LH2. Furthermore, CD measurements and sequence analysis suggest the presence of considerable beta-type in addition to alpha-helical secondary structure. Thus, the secondary and quaternary structures of this complex differ significantly from light-harvesting complexes of other purple photosynthetic bacteria. A carotenoid-protein complex is also described; it is an apparent association of three proteins and carotenoid and is closely associated with the peripheral antenna. The purple-sulfur bacteria are evolutionarily older than the relatively better characterized purple-nonsulfur organisms. The phenotypic features described here of the C. purpuratum photosynthetic apparatus are related to those of other purple bacteria and green-sulfur bacteria and may reflect the evolutionary position of this organism.lld:pubmed
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pubmed-article:8117674pubmed:authorpubmed-author:ThornberJ PJPlld:pubmed
pubmed-article:8117674pubmed:authorpubmed-author:YeatesT OTOlld:pubmed
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pubmed-article:8117674pubmed:pagination2178-84lld:pubmed
pubmed-article:8117674pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:8117674pubmed:articleTitlePurification and characterization of the peripheral antenna of the purple-sulfur bacterium Chromatium purpuratum: evidence of an unusual pigment-protein composition.lld:pubmed
pubmed-article:8117674pubmed:affiliationDepartment of Biology, University of California, Los Angeles 90024.lld:pubmed
pubmed-article:8117674pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8117674pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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