Linked Life Data

811763

Source:http://linkedlifedata.com/resource/pubmed/id/811763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-3-1
pubmed:abstractText
The synthesis of nitrogenase by intact cells of Rhodospirillum rubrum was repressed in N-free media supplemented with L-glutamine or L-asparagine, but was unaffected by the presence of L-glutamate, L-aspartate or L-histidine. Specific activities attained by cultures in supplemented media maintained under Ar-CO2 were 2 to 3 times higher than those in N-free medium under N2-CO2. A loss in total activity occurred both in cultures growing with N2 after maximum activity had been reached, and in cultures maintained under Ar when the gas phase was changed to N2. There was a rapid loss in nitrogen-fixing activity when low concentrations of NH4+, L-glutamine or L-asparagine were added to cultures with high activities, but this could be recovered in the absence of demonstrable protein synthesis. During growth, the degree of inactivation brought about by 0-5 mM-inactivator increased to 80 to 90%, and NH4+ excreted into the medium reached a maximum concentration towards the end of exponential growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
53-62
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine.
pubmed:publicationType
Journal Article