Linked Life Data

8117440

Source:http://linkedlifedata.com/resource/pubmed/id/8117440

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-4-1
pubmed:abstractText
Plasmids encoding tissue-type plasminogen activators lacking residues K296-G302 were constructed and were expressed in a Hela cell transient expression system. Conditioned media from the cultures were tested in a number of systems designed to detect function or antigen. Functional assays comprised the chromogenic substrate S2288 and three plasminogen activation assays. All t-PA variants were active in all assays but to varying degrees. The results are consistent with the conclusion that deletion of residues K296-G302 from the slowly-cleared t-PA mutant t-PA del(G) adversely affects the plasminogen activating ability of the molecule without altering the integrity of the active site. This is in contrast to the situation in native t-PA where the same mutation has little overall effect on activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1045-1056
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
269-74
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Deletion of residues K296-G302 from the slowly-cleared tissue-type plasminogen activator t-PA del (G) leads to partial loss of plasminogen activating activity.
pubmed:affiliation
Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Surrey, U.K.
pubmed:publicationType
Journal Article