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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1976-1-26
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pubmed:abstractText |
Excision-repair of DNA base damage produced by 4-nitroquinoline-1-oxide (4NQO) was compared in Escherichia coli, human cells, and mouse cells. Paper chromotography of acid hydrolysates of DNA extracted from cells treated with 3H-labeled 4NQO revealed four peaks; two kinds of 4NQO-guanine adduct, one kind of 4NQO-adenine adduct, and free 4-aminoquinoline-1-oxide (4AQO). About 80% of the initially formed 4NQO-purine adducts were excised from DNA in E. coli uvrA+ cells during 60 min postincubation, but not at all in uvrA- (excisionless for uv damage) cells. Normal human cells excised about 60% of 4NQO-purine adducts during 24 hr postincubation, but xeroderma pigmentosum (excisionless) cells did not. A mouse cell line susceptible to repair of 4NQO-induced pretransformational damage also showed excision-repair ability for the 4NQO adducts. From these and other results, we conclude that the 4NQO-purine adducts and unstable 4NQO-guanine products (which release 4AQO) are, like pyrimidine dimers, repairable by excision-repair universal among E. coli, mouse, and human being, and that unexcised ones are probably the major cause of killing, mutation, and cancer.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0090-5542
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5B
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
763-71
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:811213-4-Nitroquinoline-1-oxide,
pubmed-meshheading:811213-Cell Line,
pubmed-meshheading:811213-Cell Survival,
pubmed-meshheading:811213-DNA, Bacterial,
pubmed-meshheading:811213-DNA Repair,
pubmed-meshheading:811213-Escherichia coli,
pubmed-meshheading:811213-Kinetics,
pubmed-meshheading:811213-Molecular Weight,
pubmed-meshheading:811213-Mutation,
pubmed-meshheading:811213-Neoplasms,
pubmed-meshheading:811213-Nitroquinolines,
pubmed-meshheading:811213-Radiation Effects,
pubmed-meshheading:811213-Ultraviolet Rays
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pubmed:year |
1975
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pubmed:articleTitle |
Excision-repair of 4-nitroquinolin-1-oxide damage responsible for killing, mutation, and cancer.
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pubmed:publicationType |
Journal Article
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