Linked Life Data

8110820

Source:http://linkedlifedata.com/resource/pubmed/id/8110820

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-3-28
pubmed:abstractText
Small lipid-soluble cations, such as tetraphenylphosphonium (TPP+) and tetraphenylarsonium (TPA+) are frequently used as probes of membrane voltage (delta psi, or Vm) for small animal cells, organelles, and vesicles. Because much controversy has accompanied corresponding measurements on 'walled' eukaryotic cells (plants, fungi), we studied their transport and relation to Vm in the large-celled fungus Neurospora crassa-where Vm can readily be determined with microelectrodes-as well as in the most commonly used model eukaryotic cell, the yeast Saccharomyces cerevisiae. We found no reasonable conditions under which the distribution of TPP+ or TPA+, between the cytoplasm (i) and extracellular solution (o), can serve to estimate Vm, even roughly, in either of these organisms. When applied at probe concentrations (i.e., < or = 100 microM, which did not depolarize the cells nor deplete ATP), TPP+ stabilized at ratios (i/o) below 30 in both organisms. That would imply apparent Vm values positive to -90 mV, in the face of directly measured Vm values (in Neurospora) negative to -180 mV. When applied at moderate or high concentrations (1-30 mM), TPP+ and TPA+ induced several phases of depolarization and changes of membrane resistance (Rm), as well as depletion of cytoplasmic energy stores. Only the first phase depolarization, occurring within the perfusion-turnover time and accompanied by a nearly proportionate decline of Rm, could have resulted from TPP+ or TPA+ currents per se. And the implied currents were small. Repeated testing, furthermore, greatly reduced the depolarizing effects of these lipid-soluble ions, implicating an active cellular response to decrease membrane permeability.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
1190
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Small lipid-soluble cations are not membrane voltage probes for Neurospora or Saccharomyces.
pubmed:affiliation
Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT 06510.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't