Linked Life Data

8109722

Source:http://linkedlifedata.com/resource/pubmed/id/8109722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-3-21
pubmed:abstractText
A homogeneous immunofluorimetric technique to determine dichlorprop herbicide is described. Simultaneous determinations of both free and antibody-bound fluoresceinamine-labeled dichlorprop were carried out by measuring phase-resolved fluorescence or modulation-resolved fluorescence. The difference between the recorded values of the fluorescence lifetimes of the two species quantifies selectivity. Multifrequency heterogeneity analyses of free and bound labeled dichlorprop gave 3.68 +/- 0.03 and 4.28 +/- 0.02 ns, respectively. The differences between the phase-angles of the two species (117.5 degrees and 127.2 degrees) correspond to a difference between fluorescence lifetime values of 600 ps. This lifetime difference permits the quantitative determination of the two species simultaneously. The mean relative standard deviation of the set of three calibration curves when fitted to radioimmunoassay (chi 2 5 x 10(-4)) was 6%. The practical dynamic range was 0.1 to 100 micrograms.ml-1. On the other hand, the fractional contributions of both species, free and bound, to the total lifetime measured by demodulation, permit quantitative calculation of the two components. Both methods gave similar analytical specifications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
214
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Phase-modulation fluorescence lifetime immunoassay of dichlorprop.
pubmed:affiliation
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't