| pubmed-article:8109385 | pubmed:abstractText | Phosphorus NMR spectroscopy and HPLC analyses were made on isolated rat and mouse muscles selected for different volume fractions of the major known fiber types. We tested the hypothesis that muscle cell types at rest have intrinsically different contents of PCr, ATP and Pi. The Pi content was low and the PCr and ATP contents were high in muscles with large contents of type 2b and 2a fibers, and vice versa in muscles with large volume fraction of types 1 and 2x fibers. From the profile of these metabolites we could distinguish only two classes of fibers in the murine muscles and predict well the composition of cat muscles. For the first class, types 2a and 2b fibers, the intracellular concentrations were: ATP 8 mM; total Cr 39 mM; PCr 32 mM; Pi 0.8 mM; ADP 8 microM. For the second class, type 1 and 2x fibers, these quantities are: ATP 5 mM; TCr 23 mM; PCr 16 mM; Pi 6 mM; ADP 11 microM. Thus our results establish a new and apparently general criterion upon which to distinguish skeletal muscle cells, one based on the resting content of bioenergetically important metabolites. | lld:pubmed |
