Linked Life Data

8109385

Source:http://linkedlifedata.com/resource/pubmed/id/8109385

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-3-21
pubmed:abstractText
Phosphorus NMR spectroscopy and HPLC analyses were made on isolated rat and mouse muscles selected for different volume fractions of the major known fiber types. We tested the hypothesis that muscle cell types at rest have intrinsically different contents of PCr, ATP and Pi. The Pi content was low and the PCr and ATP contents were high in muscles with large contents of type 2b and 2a fibers, and vice versa in muscles with large volume fraction of types 1 and 2x fibers. From the profile of these metabolites we could distinguish only two classes of fibers in the murine muscles and predict well the composition of cat muscles. For the first class, types 2a and 2b fibers, the intracellular concentrations were: ATP 8 mM; total Cr 39 mM; PCr 32 mM; Pi 0.8 mM; ADP 8 microM. For the second class, type 1 and 2x fibers, these quantities are: ATP 5 mM; TCr 23 mM; PCr 16 mM; Pi 6 mM; ADP 11 microM. Thus our results establish a new and apparently general criterion upon which to distinguish skeletal muscle cells, one based on the resting content of bioenergetically important metabolites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
332
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
749-60; discussion 760-1
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Two classes of mammalian skeletal muscle fibers distinguished by metabolite content.
pubmed:affiliation
Department of Radiology, University of Washington, Seattle.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't