Linked Life Data

8107013

Source:http://linkedlifedata.com/resource/pubmed/id/8107013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-3-24
pubmed:abstractText
This study was undertaken to examine the expression and cellular location of the various cAMP-dependent protein kinase (PKA) subunits in different testicular cell types, using cDNA probes, isoenzyme-specific antibodies and activity measurements. Amounts of mRNA and protein were examined in cultured Sertoli cells, cultured peritubular cells, germ cells (pachytene spermatocytes, round spermatids), Leydig cell tumours as well as whole testes from rats of various ages. In Sertoli cells, there was a good correlation between the amount of mRNA and the respective immunoreactive proteins. In other types of cell, such as germ cells and Leydig tumour cells, this was not always the case. Large amounts of RII beta mRNA were found in Leydig tumour cells, whereas the amount of immunoreactive protein was low. Furthermore, large amounts of small-sized, germ cell-specific mRNAs for RI alpha (1.7 kb) and RII alpha (2.2 kb) were also found in the developing rat testis after 30 to 40 days of age, but the large amounts of mRNA were only partially reflected at the protein level. Pachytene spermatocytes and round spermatids were practically devoid of both RII alpha and RII beta protein. During spermatid differentiation, there was a decrease in RI alpha and an increase in RII alpha protein. Cell specific distribution of the various PKA subunits in testicular cell types is described. In some types of cell, discrepancies between mRNA and protein were demonstrated, which clearly suggest cell specific differences in translational efficiencies for some of these mRNAs, particularly the small-sized mRNAs for RI alpha and RII alpha in meiotic and post-meiotic germ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
323-34
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8107013-Aging, pubmed-meshheading:8107013-Animals, pubmed-meshheading:8107013-Blotting, Northern, pubmed-meshheading:8107013-Blotting, Western, pubmed-meshheading:8107013-Chromatography, DEAE-Cellulose, pubmed-meshheading:8107013-Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit, pubmed-meshheading:8107013-Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit, pubmed-meshheading:8107013-Cyclic AMP-Dependent Protein Kinase RIalpha Subunit, pubmed-meshheading:8107013-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:8107013-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:8107013-Isoenzymes, pubmed-meshheading:8107013-Male, pubmed-meshheading:8107013-Protein Binding, pubmed-meshheading:8107013-RNA, Messenger, pubmed-meshheading:8107013-Rats, pubmed-meshheading:8107013-Sertoli Cells, pubmed-meshheading:8107013-Spermatids, pubmed-meshheading:8107013-Spermatocytes, pubmed-meshheading:8107013-Testis
pubmed:year
1993
pubmed:articleTitle
Cellular location and age-dependent changes of the regulatory subunits of cAMP-dependent protein kinase in rat testis.
pubmed:affiliation
Institute of Medical Biochemistry, University of Oslo, Norway.
pubmed:publicationType
Journal Article