Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1994-3-24
|
| pubmed:abstractText |
Langerhans cells (LC) belong to the dendritic cell family and represent the principal antigen presenting cells populating squamous epithelia. We have reported the presence of human immunodeficiency virus Type 1 (HIV-1) proviral DNA and RNA in purified LC from the epidermis of seropositive patients. The aim of this study was to quantify HIV-1 proviral DNA in LC of infected patients using a competitive polymerase chain reaction (PCR) assay. Bulk epidermal cell (EC) suspensions were obtained from the skin of nine AIDS patients and six seronegative subjects. Purified LC and LC-depleted EC were prepared by immunomagnetic separation using an anti-CD1a monoclonal antibody. LC preparations did not contain T cells, as assessed by reverse transcription PCR analysis of the T cell receptor beta-chain gene (C region). In addition, no CD14+ cells could be detected in LC fractions by immunostaining of cytospin preparations. To quantify HIV-1 DNA, a new competitive PCR system was devised using SK145/150 as primers (gag) and a competitor plasmid DNA with a modified sequence (209 instead of 142 bp). The number of HIV-1 DNA copies found in the LC of AIDS patients ranged from 107 to 3,645/10(5) LC. In contrast, LC-depleted EC from the same subjects were all negative. The results indicate that in AIDS patients the frequency of infected LC is comparable to that reported for peripheral blood CD4+ T cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0894-9255
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
230-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8106964-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:8106964-Adult,
pubmed-meshheading:8106964-Aged,
pubmed-meshheading:8106964-Binding, Competitive,
pubmed-meshheading:8106964-DNA, Viral,
pubmed-meshheading:8106964-Female,
pubmed-meshheading:8106964-HIV-1,
pubmed-meshheading:8106964-HLA-DR Antigens,
pubmed-meshheading:8106964-Humans,
pubmed-meshheading:8106964-Immunomagnetic Separation,
pubmed-meshheading:8106964-Langerhans Cells,
pubmed-meshheading:8106964-Male,
pubmed-meshheading:8106964-Middle Aged,
pubmed-meshheading:8106964-Plasmids,
pubmed-meshheading:8106964-Polymerase Chain Reaction,
pubmed-meshheading:8106964-Proviruses,
pubmed-meshheading:8106964-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8106964-Rosette Formation,
pubmed-meshheading:8106964-Transcription, Genetic
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Quantitation by competitive PCR of HIV-1 proviral DNA in epidermal Langerhans cells of HIV-infected patients.
|
| pubmed:affiliation |
Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|