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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1994-3-22
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pubmed:databankReference |
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pubmed:abstractText |
We have analyzed the gene structure that gives rise to tissue-specific isoforms of the Na/Ca exchanger. Five distinct isoforms of the Na/Ca exchanger from rabbit brain, kidney, and heart have been identified previously to which we now add a new brain isoform. Reverse-transcribed polymerase chain reaction, library screening, and sequence analysis of cDNA coding regions indicate that the only significant alteration of the Na/Ca exchanger cDNA in rabbit brain, kidney, and heart isoforms is located in the carboxyl end of the putative intracellular loop of the protein, a region recently linked to ionic and metabolic regulation of the Na/Ca exchanger. Additionally, we find that the Na/Ca exchanger isoforms found in lung and skeletal muscle may arise from among these same six isoforms. Examination of the gene structure of the Na/Ca exchanger in rabbit indicates how the single gene that encodes for the Na/Ca exchanger is alternatively spliced to give rise to the five rabbit isoforms. Specifically, sequence analysis of the intron-exon boundaries reveals the presence of two "mutually exclusive" exons in conjunction with four "cassette" exons in the region of the Na/Ca exchanger gene that codes for the carboxyl end of the predicted intracellular loop region. This unusual arrangement of exons in the Na/Ca exchanger gene could allow for the generation of up to 32 different Na/Ca exchanger mRNAs and accounts for the isoforms identified to date.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5145-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8106495-Alternative Splicing,
pubmed-meshheading:8106495-Amino Acid Sequence,
pubmed-meshheading:8106495-Animals,
pubmed-meshheading:8106495-Base Sequence,
pubmed-meshheading:8106495-Brain,
pubmed-meshheading:8106495-Calcium,
pubmed-meshheading:8106495-Carrier Proteins,
pubmed-meshheading:8106495-DNA,
pubmed-meshheading:8106495-DNA, Complementary,
pubmed-meshheading:8106495-DNA Primers,
pubmed-meshheading:8106495-Exons,
pubmed-meshheading:8106495-Genetic Variation,
pubmed-meshheading:8106495-Genomic Library,
pubmed-meshheading:8106495-Introns,
pubmed-meshheading:8106495-Kidney,
pubmed-meshheading:8106495-Molecular Sequence Data,
pubmed-meshheading:8106495-Myocardium,
pubmed-meshheading:8106495-Organ Specificity,
pubmed-meshheading:8106495-Polymerase Chain Reaction,
pubmed-meshheading:8106495-Protein Biosynthesis,
pubmed-meshheading:8106495-RNA, Messenger,
pubmed-meshheading:8106495-Rabbits,
pubmed-meshheading:8106495-Sequence Homology, Amino Acid,
pubmed-meshheading:8106495-Sodium,
pubmed-meshheading:8106495-Sodium-Calcium Exchanger
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pubmed:year |
1994
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pubmed:articleTitle |
Mutually exclusive and cassette exons underlie alternatively spliced isoforms of the Na/Ca exchanger.
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pubmed:affiliation |
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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