Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-3-22
pubmed:abstractText
The influence of the insulin secretagogues, glucose and K+, to activate the multifunctional, Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in isolated rat pancreatic islets has been examined. Glucose (28 mM) and K+ (40 mM) were demonstrated to induce a 1.89 +/- 0.19- and 1.75 +/- 0.15-fold increase, respectively, in phosphorylation of a subunit of CaM kinase II immunoprecipitated by an anti-CaM kinase II alpha antibody. In intact islets, glucose and K+ also induced the generation of an autonomous, Ca2+/calmodulin-independent protein kinase II activity characteristic of autophosphorylated enzyme. Maximal activation, 2.9 +/- 0.2- and 3.0 +/- 0.5-fold for glucose and K+, respectively, relative to basal glucose control, was achieved at 2.5-5 min followed by a decline to near basal levels by 20 min. Glucose induced the production of autonomous CaM kinase II activity that, in terms of -fold stimulation, correlated closely with the extent of insulin release over a glucose concentration range of 3-28 mM. This stimulated activity was completely prevented by an inhibitor of glucose metabolism, mannoheptulose. These data demonstrate that the exposure of islets to stimulatory glucose concentrations activates CaM kinase II. The close correlation of enzyme activation with insulin secretion is consistent with the hypothesis that CaM kinase II plays an important role in the regulation of insulin secretion or related beta-cell processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4947-52
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8106469-Amino Acid Sequence, pubmed-meshheading:8106469-Animals, pubmed-meshheading:8106469-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:8106469-Cells, Cultured, pubmed-meshheading:8106469-Enzyme Activation, pubmed-meshheading:8106469-Enzyme Induction, pubmed-meshheading:8106469-Glucose, pubmed-meshheading:8106469-Insulin, pubmed-meshheading:8106469-Islets of Langerhans, pubmed-meshheading:8106469-Isoenzymes, pubmed-meshheading:8106469-Kinetics, pubmed-meshheading:8106469-Male, pubmed-meshheading:8106469-Molecular Sequence Data, pubmed-meshheading:8106469-Molecular Weight, pubmed-meshheading:8106469-Oligopeptides, pubmed-meshheading:8106469-Peptides, pubmed-meshheading:8106469-Potassium, pubmed-meshheading:8106469-Rats, pubmed-meshheading:8106469-Rats, Wistar
pubmed:year
1994
pubmed:articleTitle
Glucose activates the multifunctional Ca2+/calmodulin-dependent protein kinase II in isolated rat pancreatic islets.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of North Texas Health Science Center at Fort Worth 76107-2699.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't