Linked Life Data

8106396

Source:http://linkedlifedata.com/resource/pubmed/id/8106396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-3-18
pubmed:abstractText
In order to identify protein complexes consisting of the proteasome and specific proteasome regulators, crude soluble lysates of red blood cells were fractionated by gel filtration chromatography and by velocity sedimentation centrifugation. The fractionated lysates were then tested for the relative distribution of proteasome activity, proteasome protein, and protein of a known proteasome activator, PA28. At least two proteasome complexes containing PA28 were identified. One of these complexes had an apparent molecular weight of approximately 1,750,000, and appeared to have much more proteasome activity than could be accounted for by its relative concentrations of proteasome and PA28 protein. We hypothesized that this complex contained another activator of the proteasome, and we sought to purify this activator from extracts of red blood cells. A proteasome activator with an apparent molecular weight of approximately 700,000 was identified, purified, and characterized. This activator, termed PA700, greatly stimulated the peptidase activities of the proteasome in an ATP-dependent fashion. PA700 was composed of about 16 polypeptides ranging in molecular weight from 20,000 to 100,000. The ATP-dependent activation of the proteasome by PA700 was closely linked to the formation of a high molecular weight complex that required no additional ATP for activated proteolysis. These results indicate that PA700 is a regulatory protein of the proteasome and is a component of at least one high molecular weight proteasome-containing complex occurring in cell extracts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3539-47
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8106396-Adenine Nucleotides, pubmed-meshheading:8106396-Adenosine Triphosphate, pubmed-meshheading:8106396-Amino Acid Sequence, pubmed-meshheading:8106396-Animals, pubmed-meshheading:8106396-Blood Proteins, pubmed-meshheading:8106396-Cattle, pubmed-meshheading:8106396-Chromatography, pubmed-meshheading:8106396-Chromatography, DEAE-Cellulose, pubmed-meshheading:8106396-Chromatography, Gel, pubmed-meshheading:8106396-Cysteine Endopeptidases, pubmed-meshheading:8106396-Durapatite, pubmed-meshheading:8106396-Enzyme Activation, pubmed-meshheading:8106396-Erythrocytes, pubmed-meshheading:8106396-Kinetics, pubmed-meshheading:8106396-Molecular Sequence Data, pubmed-meshheading:8106396-Molecular Weight, pubmed-meshheading:8106396-Multienzyme Complexes, pubmed-meshheading:8106396-Oligopeptides, pubmed-meshheading:8106396-Proteasome Endopeptidase Complex, pubmed-meshheading:8106396-Proteins, pubmed-meshheading:8106396-Ribonucleotides, pubmed-meshheading:8106396-Substrate Specificity
pubmed:year
1994
pubmed:articleTitle
Identification, purification, and characterization of a high molecular weight, ATP-dependent activator (PA700) of the 20 S proteasome.
pubmed:affiliation
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't