Linked Life Data

8106326

Source:http://linkedlifedata.com/resource/pubmed/id/8106326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-3-21
pubmed:abstractText
Chemoreceptor Trg and osmosensor EnvZ of Escherichia coli share a common transmembrane organization but have essentially unrelated primary structures. We created a hybrid gene coding for a protein in which Trg contributed its periplasmic and transmembrane domains as well as a short cytoplasmic segment and EnvZ contributed its cytoplasmic kinase/phosphatase domain. Trz1 transduced recognition of sugar-occupied, ribose-binding protein by its periplasmic domain into activation of its cytoplasmic kinase/phosphatase domain as assessed in vivo by using an ompC-lacZ fusion gene. Functional coupling of sugar-binding protein recognition to kinase/phosphatase activity indicates shared features of intramolecular signalling in the two parent proteins. In combination with previous documentation of transduction of aspartate recognition by an analogous fusion protein created from chemoreceptor Tar and EnvZ, the data indicate a common mechanism of transmembrane signal transduction by chemoreceptors and EnvZ. Signalling through the fusion proteins implies functional interaction between heterologous domains, but the minimal sequence identity among relevant segments of EnvZ, Tar, and Trg indicates that the link does not require extensive, specific interactions among side chains. The few positions of identity in those three sequences cluster in transmembrane segment 1 and the short chemoreceptor sequence in the cytoplasmic part of the hybrid proteins. These regions may be particularly important in physical and functional coupling. The specific cellular conditions necessary to observe ligand-dependent activation of Trz1 can be understood in the context of the importance of phosphatase control in EnvZ signalling and limitations on maximal receptor occupancy in binding protein-mediated recognition.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1482126, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1518809, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1537797, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1592821, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1662380, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-1883200, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2104606, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2254280, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2277041, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2476847, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2556636, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2656684, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2663643, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2668953, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2677004, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2824492, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2834370, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2846509, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2848552, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-2997120, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3049535, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3049536, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3052756, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3056621, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3087946, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3139636, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-385590, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3883356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-3894359, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-6327617, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-8389884, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-8393937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-8416890, http://linkedlifedata.com/resource/pubmed/commentcorrection/8106326-8491182
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribose, http://linkedlifedata.com/resource/pubmed/chemical/Trg protein, E coli, http://linkedlifedata.com/resource/pubmed/chemical/envZ protein, E coli
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
176
pubmed:geneSymbol
envZ, lacZ, ompC, trg, trz1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1157-63
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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