Linked Life Data

8106089

Source:http://linkedlifedata.com/resource/pubmed/id/8106089

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-12-14
pubmed:abstractText
Ganglioside (GM1) modulation of CD4 off the surface of T lymphocytes defined functions of the CD4 molecule during signal transduction through the T cell receptor (TCR)/CD3 complex. Antibody cross-linking of CD3 alone (3 x 3) stimulated phospholipase C (PLC) activity, rapid Ca2+ flux, and protein phosphorylations in freshly isolated human T lymphocytes. Antibody cross-linking of CD4 and CD3 (3 x 4) stimulated greater signaling than that caused by 3 x 3. Cross-linking CD4 alone did not stimulate these signaling processes. GM1-modulation of CD4 from the cell surface blocked all aspects of the augmented signaling imparted by CD4 co-modulation with CD3. In comparison, pretreatment with the protein tyrosine kinase inhibitor genistein inhibited 3 x 4-stimulated PLC activity and protein phosphorylation but not Ca2+ flux. Antibody cross-linking of the tyrosine phosphatase CD45 with 3 x 4 (3 x 4 x 45) also inhibited CD4-augmented phosphorylations and like genistein did not reduce Ca2+ levels. In conclusion, these data demonstrate that CD4 can augment signal transduction through the TCR/CD3 complex by its physical proximity to CD3. TCR/CD3-signaling augmentation by CD4 stimulated protein tyrosine kinases and PLC activities but stimulated intracellular Ca2+ flux through an independent mechanism(s).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0968-8773
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-65
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8106089-Antigens, CD4, pubmed-meshheading:8106089-CD4-Positive T-Lymphocytes, pubmed-meshheading:8106089-Calcium, pubmed-meshheading:8106089-Enzyme Activation, pubmed-meshheading:8106089-G(M1) Ganglioside, pubmed-meshheading:8106089-Genistein, pubmed-meshheading:8106089-Isoflavones, pubmed-meshheading:8106089-Lymphocyte Activation, pubmed-meshheading:8106089-Muromonab-CD3, pubmed-meshheading:8106089-Phosphatidylinositol Diacylglycerol-Lyase, pubmed-meshheading:8106089-Phosphoric Diester Hydrolases, pubmed-meshheading:8106089-Phosphorylation, pubmed-meshheading:8106089-Protein Processing, Post-Translational, pubmed-meshheading:8106089-Protein-Tyrosine Kinases, pubmed-meshheading:8106089-Receptor-CD3 Complex, Antigen, T-Cell, pubmed-meshheading:8106089-Signal Transduction
pubmed:year
1993
pubmed:articleTitle
Ganglioside (GM1) distinguishes the effects of CD4 on signal transduction through the TCR/CD3 complex in human lymphocytes.
pubmed:affiliation
Neuroimmunology Research Laboratory, Veterans Administration Medical Center, Portland, OR 97207.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't