Linked Life Data

8105445

Source:http://linkedlifedata.com/resource/pubmed/id/8105445

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1993-11-10
pubmed:abstractText
Na pump current was measured in rat ventricular myocytes to determine if beta-adrenergic stimulation can directly modulate Na,K-ATPase activity. Enzymatically-isolated heart cells were voltage-clamped with a single patch electrode and Na pump current was briefly activated by rapidly increasing extracellular [K+] from 0 to 15 mM for 3-5 s after other ionic currents were blocked or inactivated. The salt solution in the voltage-clamping electrode included (in mM): (1) 100 Na+, 10 EGTA, (2) 5 Na+, 10 EGTA, or (3) 100 Na+, 7.5 Ca2+, 10 EGTA (free [Ca2+] = 480 nM). With all three electrode solutions, Na pump current was not significantly changed after 2-4 min in the presence of 10 microM isoprenaline. beta-adrenergic pathways were still intact as evidenced by the two-fold increase in Cd(2+)-sensitive Ba2+ current through calcium channels that was observed in the presence of isoprenaline. Thus, beta-adrenergic stimulation does not appear to directly regulate Na,K-ATPase activity in rat ventricular myocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
424
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-3
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Beta-adrenergic stimulation does not regulate Na pump function in voltage-clamped ventricular myocytes of the rat heart.
pubmed:affiliation
Bockus Research Institute, Graduate Hospital, Philadelphia, PA 19146.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't