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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1976-1-8
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pubmed:abstractText |
Sheep red blood cells were stabilized with sulfosalicylic acid and sensitized with purified horse-IgG by the action of glutardialdehyde. The cells were used in an indirect microhemmagglutination test with serum samples from 12 rabbits immunized with horse-IgG and complete Freund's adjuvant as well as from 10 control animals. 32 human serum samples were examined, 6 of them from patients with established horse protein allergy. The results were compared to those of a standard method of indirect hemagglutination using fresh human erythrocytes (group O Rh-), an active hemagglutination test against horse erythrocytes, Ouchterlony's immunodiffusion and skin tests with horse-IgG. The method proved to be very sensitive. The results correlated well with those of the other immunological techniques. The stable sensitized cells did not lose sensitivity after 1 year of storage at 4 degrees C.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0020-5915
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:810439-Animals,
pubmed-meshheading:810439-Antibodies,
pubmed-meshheading:810439-Drug Storage,
pubmed-meshheading:810439-Erythrocytes,
pubmed-meshheading:810439-Glutaral,
pubmed-meshheading:810439-Hemagglutination Tests,
pubmed-meshheading:810439-Horses,
pubmed-meshheading:810439-Humans,
pubmed-meshheading:810439-Hypersensitivity,
pubmed-meshheading:810439-Immunodiffusion,
pubmed-meshheading:810439-Immunoglobulin G,
pubmed-meshheading:810439-Injections, Intramuscular,
pubmed-meshheading:810439-Rabbits,
pubmed-meshheading:810439-Sheep,
pubmed-meshheading:810439-Skin Tests
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pubmed:year |
1976
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pubmed:articleTitle |
Use of indirect microhemagglutination test with stable sensitized red cells in the detection of horse protein allergy.
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pubmed:publicationType |
Journal Article,
Comparative Study
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