Linked Life Data

810350

Source:http://linkedlifedata.com/resource/pubmed/id/810350

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1976-1-14
pubmed:abstractText
In the chain-lengthening of the oligosaccharide chains of endogenous dolichol-diphosphate oligosaccharides (DOL-P-P-oligosaccharides) by a pig liver microsomal preparation dolichol-monophosphate mannose (Dol-P-Man) was a more efficient donor of mammose (a maximum of 35% transferred by 5 min) than was GDP-Man (reaching 16% by 45 min). The effects of an excess of GDP, an excess of GDP-Man, a lack of Mn2+ and an excess of EDTA showed that the transfer from GDP-Man was via Dol-P-Man. The evidence also indicated the presence of two pools of Dol-P-Man one of which was difficult to extract and which was possibly closely associated with the Dol-P-P-oligosaccharides and the appropriate transferase. After 1 h of incubation transfer of 14C to 'insoluble polymer' from GDP-[14C]Man, Dol-P-[14C]Man and Dol-P-P-[14C]oligosaccharides reached approximately 3%, 3% and 13% respectively, of that available. The result of adding excess unlabelled GDP-Man to an incubation with GDP-[14C]Man in progressconfirmed the sequence GDP-Man leads to Dol-P-Man leads to Dol-P-P-oligosaccharide leads to insoluble polymer. Solubilisation with sodium dodecyl sulphate of the radioactive 'insoluble polymer' followed by gel chromatography showed the presence of radioactive glycoprotein and oligosaccharide when either GDP-[14C]Man or Dol-P-P-[14C]oligosaccharide was used as donor. The proportion of oligosaccharide formed rose sharply when excess EDTA was present and GDP-[14C]Man was the donor. Under these conditions the oligosaccharide contained 5--6 units and all of the radioactivity could be released by alpha-mannosidase. The glycoprotein was susceptible to proteolysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-9
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
The mannosylation of dolichol-diphosphate oligosaccharides in relation to the formation of oligosaccharides and glycoproteins in pig-liver endoplasmic reticulum.
pubmed:publicationType
Journal Article