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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1993-9-1
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pubmed:abstractText |
1. Serum-containing media without additional hormones failed to support the differentiation of porcine SV cells while hormone-supplemented serum-free medium (ITTC) stimulated differentiation as indicated by higher GPDH specific activity. 2. All sera tested stimulated proliferation and inhibited porcine SV cell differentiation to some degree; fetal calf and rat sera were more inhibitory than calf and pig sera. 3. Morphologically, in serum-free medium porcine SV cells developed into individual adipocytes while in all serum-containing media they formed fat cell clusters. 4. Insulin and hydrocortisone in serum-free medium were important factors in SV cell differentiation while transferrin, T3, and fibroblast growth factor (FGF) were less important.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
|
pubmed:author | |
pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-92
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8101780-Adipose Tissue,
pubmed-meshheading:8101780-Animals,
pubmed-meshheading:8101780-Blood,
pubmed-meshheading:8101780-Cell Differentiation,
pubmed-meshheading:8101780-Cell Division,
pubmed-meshheading:8101780-Cells, Cultured,
pubmed-meshheading:8101780-Culture Media, Serum-Free,
pubmed-meshheading:8101780-Fibroblast Growth Factors,
pubmed-meshheading:8101780-Insulin,
pubmed-meshheading:8101780-Stromal Cells,
pubmed-meshheading:8101780-Swine,
pubmed-meshheading:8101780-Transferrin
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pubmed:year |
1993
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pubmed:articleTitle |
Effect of serum on differentiation of porcine adipose stromal-vascular cells in primary culture.
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pubmed:affiliation |
Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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