Linked Life Data

8100535

Source:http://linkedlifedata.com/resource/pubmed/id/8100535

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-8-4
pubmed:abstractText
A cell separation method was developed for studies of the growth kinetics of rat mammary epithelial cell (RMEC) subpopulations in grafts and in culture in vitro. By flow cytometry of RMEC stained with fluorescein isothiocyanate-peanut agglutinin and phycoerythrin-anti-Thy-1.1 monoclonal antibody, we could distinguish four cell subpopulations from primary cultures of 7- to 8-week-old F344 female rat mammary glands: both negative (B-), PNA+, Thy-1.1+, and both positive (B+). We studied the growth patterns of these subpopulations in vitro for 1 to 14 days in complete hormone medium (CHM) containing 10% fetal bovine serum and prolactin, 17 beta-estradiol, cortisol, progesterone, and insulin. The fractions of PNA+ and B- cells steadily decreased with time in culture. The fraction of Thy-1.1+ cells steadily increased with time in culture. There were small numbers of B+ cells. We grafted RMEC in hyperprolactinemic recipient rats. The mean numbers of transplanted cells required to produce at least one alveolar unit in 50% of the graft sites (AD50 values) are inversely related to the clonogenic fractions. AD50s of RMEC are as follows: unsorted mammary cells cultured in CHM for 1 to 4 days, approximately 220; unsorted cells cultured in CHM for 7 days, approximately 550; sorted PNA+ RMEC from outgrowths of 3-day cultures, approximately 82; B+, approximately 350; B-, approximately 545; Thy-1.1+, approximately 9372. We conclude that the PNA+ cell subpopulation includes most of the clonogenic cells. Thy-1.1+ cells appear to be terminally differentiated; they are likely to be myoepithelial cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
207
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
74-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Characterization of rat mammary epithelial cell subpopulations by peanut lectin and anti-Thy-1.1 antibody and study of flow-sorted cells in vivo.
pubmed:affiliation
Department of Human Oncology, University of Wisconsin Comprehensive Cancer Center, Madison 53792.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.