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rdf:type |
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lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0010031,
umls-concept:C0022563,
umls-concept:C0033684,
umls-concept:C0033692,
umls-concept:C0126273,
umls-concept:C0444669,
umls-concept:C0678594,
umls-concept:C1519249,
umls-concept:C1710548,
umls-concept:C1882932
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pubmed:issue |
16
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pubmed:dateCreated |
1993-7-7
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pubmed:databankReference |
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pubmed:abstractText |
Amino acid sequence from tryptic peptides of three different bovine corneal keratan sulfate proteoglycan (KSPG) core proteins (designated 37A, 37B, and 25) showed similarities to the sequence of a chicken KSPG core protein lumican. Bovine lumican cDNA was isolated from a bovine corneal expression library by screening with chicken lumican cDNA. The bovine cDNA codes for a 342-amino acid protein, M(r) 38,712, containing amino acid sequences identified in the 37B KSPG core protein. The bovine lumican is 68% identical to chicken lumican, with an 83% identity excluding the N-terminal 40 amino acids. Location of 6 cysteine and 4 consensus N-glycosylation sites in the bovine sequence were identical to those in chicken lumican. Bovine lumican had about 50% identity to bovine fibromodulin and 20% identity to bovine decorin and biglycan. About two-thirds of the lumican protein consists of a series of 10 amino acid leucine-rich repeats that occur in regions of calculated high beta-hydrophobic moment, suggesting that the leucine-rich repeats contribute to beta-sheet formation in these proteins. Sequences obtained from 37A and 25 core proteins were absent in bovine lumican, thus predicting a unique primary structure and separate mRNA for each of the three bovine KSPG core proteins.
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pubmed:grant |
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pubmed:keyword |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11874-80
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8099356-Amino Acid Sequence,
pubmed-meshheading:8099356-Animals,
pubmed-meshheading:8099356-Base Sequence,
pubmed-meshheading:8099356-Cattle,
pubmed-meshheading:8099356-Cells, Cultured,
pubmed-meshheading:8099356-Chickens,
pubmed-meshheading:8099356-Chondroitin Sulfate Proteoglycans,
pubmed-meshheading:8099356-Cornea,
pubmed-meshheading:8099356-DNA,
pubmed-meshheading:8099356-Gene Library,
pubmed-meshheading:8099356-Keratan Sulfate,
pubmed-meshheading:8099356-Male,
pubmed-meshheading:8099356-Molecular Sequence Data,
pubmed-meshheading:8099356-Mutagenesis,
pubmed-meshheading:8099356-Orchiectomy,
pubmed-meshheading:8099356-Peptide Fragments,
pubmed-meshheading:8099356-Plasmids,
pubmed-meshheading:8099356-Poly A,
pubmed-meshheading:8099356-Protein Structure, Secondary,
pubmed-meshheading:8099356-RNA, Messenger,
pubmed-meshheading:8099356-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8099356-Restriction Mapping,
pubmed-meshheading:8099356-Sequence Deletion,
pubmed-meshheading:8099356-Sequence Homology, Amino Acid,
pubmed-meshheading:8099356-Trypsin
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pubmed:year |
1993
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pubmed:articleTitle |
Sequence and structural implications of a bovine corneal keratan sulfate proteoglycan core protein. Protein 37B represents bovine lumican and proteins 37A and 25 are unique.
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pubmed:affiliation |
Division of Biology, Kansas State University, Manhattan 66506.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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