Linked Life Data

8099284

Source:http://linkedlifedata.com/resource/pubmed/id/8099284

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-7-1
pubmed:abstractText
Recently it has become clear that alpha 2-adrenergic receptors (alpha 2AR) functionally couple to Gs as well as Gi, thus inducing a complex modulation of adenylyl cyclase activity. It is unknown whether alpha 2AR-Gs coupling undergoes agonist-promoted desensitization. Therefore, in CHO cells expressing the three cloned human alpha 2AR subtypes (alpha 2C10, alpha 2C4, and alpha 2C2), we assessed the ability of alpha 2AR-mediated stimulation of adenylyl cyclase activity to undergo short-term agonist-promoted desensitization. To isolate alpha 2AR-Gs coupling, cells were pretreated with pertussis toxin, which ablates Gi coupling. Following agonist exposure, both alpha 2C10- and alpha 2C2-mediated stimulation of adenylyl cyclase activity underwent marked desensitization. In distinct contrast, alpha 2C4-mediated stimulation of adenylyl cyclase activity underwent no agonist-promoted desensitization. Thus, alpha 2AR-Gs coupling undergoes agonist-promoted desensitization and does so in a subtype-selective manner.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-23
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Functional alpha 2-adrenergic receptor-Gs coupling undergoes agonist-promoted desensitization in a subtype-selective manner.
pubmed:affiliation
Department of Medicine (Pulmonary), University of Cincinnati College of Medicine, OH 45267.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.