Linked Life Data

8097799

Source:http://linkedlifedata.com/resource/pubmed/id/8097799

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-6-2
pubmed:abstractText
In order to obtain a detailed understanding of the chemical identity of callosal neurons and of their synaptic targets during development of the rat, a technique was developed combining anterograde and retrograde transport of the carbocyanine dye, DiI, previously applied in living or fixed tissue with conventional immunocytochemistry for peptides. It is reported here that photoconversion of the fluorescent DiI label to a stable diaminobenzidine reaction product is fully compatible with the application of the most widely used immunocytochemical techniques peroxidase-antiperoxidase (PAP) or avidin-biotin (ABC) on the same tissue section, for correlated light and electron microscopic studies. Advantages of this double-labeling procedure over previously described techniques which permit concurrent visualization of projection systems and chemically defined neuronal elements are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-8
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
DiI labeling combined with conventional immunocytochemical techniques for correlated light and electron microscopic studies.
pubmed:affiliation
Department of Anatomy, Veterinary School, University of Thessaloniki, Macedonia, Greece.
pubmed:publicationType
Journal Article