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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8 Pt 1
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pubmed:dateCreated |
1993-5-12
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pubmed:abstractText |
The zeta-subunit of the TCR binds GTP and is a well characterized substrate for a TCR-activated tyrosine kinase. To examine the possible coupling of GTP-binding to zeta with TCR-mediated signal transduction, a mutant (termed J32-3.2) of the T cell line Jurkat (J32) was used. Anti-TCR/CD3 stimulation of the TCR/CD3+ J32-3.2 cells resulted in a weak stimulation of both the phosphatidyl inositol and tyrosine kinase signal transduction pathways, as measured by changes in the level of free intracellular calcium, tyrosine phosphorylation of TCR-zeta, CD3-epsilon and ZAP-70, p56lck, or p59fyn tyrosine kinase activity and IL-2 gene activation. The impaired responsiveness of J32-3.2 cells to anti-TCR/CD3 mAb correlated with a low basal level of GTP-binding to zeta. Furthermore, in J32-3.2 cells TCR activation by antibody ligation caused a weaker increase in GTP-binding to the zeta-chain, as compared with that of wild-type J32 cells, which indicates for the first time that GTP-binding to zeta can be modulated by extracellular signals and suggest that the role of GTP-binding to zeta is to couple the TCR to intracellular signal transduction mechanisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/antigen T cell receptor, zeta chain
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3230-42
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8096851-Amino Acid Sequence,
pubmed-meshheading:8096851-Antibodies, Monoclonal,
pubmed-meshheading:8096851-Antigens, CD2,
pubmed-meshheading:8096851-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:8096851-Base Sequence,
pubmed-meshheading:8096851-DNA,
pubmed-meshheading:8096851-Guanosine Triphosphate,
pubmed-meshheading:8096851-Humans,
pubmed-meshheading:8096851-Interleukin-2,
pubmed-meshheading:8096851-Membrane Proteins,
pubmed-meshheading:8096851-Molecular Sequence Data,
pubmed-meshheading:8096851-Protein Binding,
pubmed-meshheading:8096851-Protein-Tyrosine Kinases,
pubmed-meshheading:8096851-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:8096851-Receptors, Antigen, T-Cell,
pubmed-meshheading:8096851-Receptors, Immunologic,
pubmed-meshheading:8096851-Signal Transduction,
pubmed-meshheading:8096851-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
Coupling of GTP-binding to the T cell receptor (TCR) zeta-chain with TCR-mediated signal transduction.
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pubmed:affiliation |
Division of Immunology, Beth Israel Hospital, Boston, MA 02215.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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