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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-4-22
|
| pubmed:abstractText |
Lung-tumor cells from pleural effusion of four refractory patients and in cell lines established from them were analyzed for anthracycline retention, cytotoxicity, and MDR-1 gene and P-glycoprotein expression. Murine leukemic P388 and doxorubicin-resistant P388/R84 lines were used as controls. The 50% growth-inhibitory concentration (IC50) for doxorubicin among lung-tumor lines varied from 0.16 to 0.31 microM in soft agar. Heterogeneity in doxorubicin or daunorubicin retention and response to the efflux-blocking action of 25 microM prochlorperazine was noted in pleural effusion of FCCL-1, -4, and -8. Among the cell lines established, an efflux-blocking effect in a subpopulation was noticed only in FCCL-1 and -4. Although the MDR-1 gene was present in all cell lines, including P388, its expression was pronounced only in P388/R84 and FCCL-1. In situ hybridization of antisense RNA probe to tumor cells showed high heterogeneity for MDR-1 message in the human lung-tumor cells as compared with the murine cells. Northern and slot blot hybridization confirmed in situ hybridization in lines with high levels of MDR-1 expression. The synthesis of MDR-1 mRNA and P-glycoprotein in tumor lines was correlated. The results suggest that because of extensive tumor-cell heterogeneity in human tumors, monitoring of MDR expression by in situ hybridization, quantitation of P-glycoprotein content by laser flow cytometry (and/or immunohistochemical methods), and drug efflux (by laser flow cytometry) may be the best ways to monitor multidrug resistance in human tumors.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/P-Glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0344-5704
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
31
|
| pubmed:geneSymbol |
MDR-1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
431-41
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8095859-Adult,
pubmed-meshheading:8095859-Aged,
pubmed-meshheading:8095859-Aged, 80 and over,
pubmed-meshheading:8095859-Animals,
pubmed-meshheading:8095859-Doxorubicin,
pubmed-meshheading:8095859-Drug Resistance,
pubmed-meshheading:8095859-Female,
pubmed-meshheading:8095859-Gene Expression,
pubmed-meshheading:8095859-Humans,
pubmed-meshheading:8095859-Leukemia P388,
pubmed-meshheading:8095859-Lung Neoplasms,
pubmed-meshheading:8095859-Male,
pubmed-meshheading:8095859-Membrane Glycoproteins,
pubmed-meshheading:8095859-Mice,
pubmed-meshheading:8095859-Middle Aged,
pubmed-meshheading:8095859-Neoplasm Proteins,
pubmed-meshheading:8095859-P-Glycoprotein,
pubmed-meshheading:8095859-RNA, Messenger,
pubmed-meshheading:8095859-RNA, Neoplasm,
pubmed-meshheading:8095859-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
MDR-1 gene expression, anthracycline retention and cytotoxicity in human lung-tumor cells from refractory patients.
|
| pubmed:affiliation |
Department of Radiation Oncology, University of Miami Medical School, FL 33101.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports
|