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rdf:type |
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lifeskim:mentions |
umls-concept:C0004371,
umls-concept:C0004595,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0026882,
umls-concept:C0029073,
umls-concept:C0040649,
umls-concept:C0185117,
umls-concept:C0205164,
umls-concept:C0392760,
umls-concept:C0815304,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1993-2-25
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pubmed:databankReference |
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pubmed:abstractText |
Transcription of the major Bacillus subtilis autolysin gene (cwlB) was investigated. Deletion of the region upstream of the gene cluster lppX-cwbA-cwlB led to a loss of promoter activity. Primer extension analysis suggested that the cwlB operon is transcribed by E sigma D and E sigma A, the former transcripts being predominants at the exponential growth phase. Expression of the lppX-lacZ fusion gene was reduced by about 90% in a sigD-null mutant. A sin (flaD1) mutation caused a severe defect in transcription of the lppX-cwbA-cwlB operon. The sin (flaD1) mutation also reduced expression of a sigD-lacZ fusion gene constructed in the B. subtilis chromosome. Since the sigD-null mutant exhibits motility and autolysin deficiencies and filamentation, similar phenotypes in the sin (flaD1) mutant may be caused by reduction in expression of the sigma D protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1356138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1592811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1592812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-16561900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1674525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1682302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1683402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1688843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1698762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1809831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1898931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1901557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1906467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1908396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-1943994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2111808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2121898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2127796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2428811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2579378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2644646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2832368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-2906087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-3096962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-3112521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-3123142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-3148618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-334155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-3926750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-4956288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-6150066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-803484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-803507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-809421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-809432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-817719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-821929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8093697-827683
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
175
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pubmed:geneSymbol |
cwbA,
cwlB,
lppX,
sigD
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
795-801
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8093697-Amino Acid Sequence,
pubmed-meshheading:8093697-Bacillus subtilis,
pubmed-meshheading:8093697-Base Sequence,
pubmed-meshheading:8093697-Cloning, Molecular,
pubmed-meshheading:8093697-DNA-Binding Proteins,
pubmed-meshheading:8093697-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8093697-Molecular Sequence Data,
pubmed-meshheading:8093697-N-Acetylmuramoyl-L-alanine Amidase,
pubmed-meshheading:8093697-Operon,
pubmed-meshheading:8093697-Promoter Regions, Genetic,
pubmed-meshheading:8093697-RNA, Messenger,
pubmed-meshheading:8093697-Repressor Proteins,
pubmed-meshheading:8093697-Restriction Mapping,
pubmed-meshheading:8093697-Sigma Factor,
pubmed-meshheading:8093697-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation.
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pubmed:affiliation |
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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