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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1994-10-18
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pubmed:databankReference | |
pubmed:abstractText |
The nucleotide sequence of the Bacteroides fragilis neuraminidase-encoding gene (nanH) and its flanking regions was determined. The B. fragilis nanH open reading frame (ORF) of 1632 nucleotides encoded a neuroaminidase of 544 amino acid residues with a calculated molecular mass of 59.51 kDa. The amino acid sequence at the N-terminus of the deduced protein exhibited the common features of procaryotic signal sequences. The Asp box sequence, Ser-X-Asp-X-Gly-X-Thr-Trp, which is conserved among bacterial neuraminidases, was found at five positions in the deduced amino acid sequence. A single transcription start point was identified 88 nucleotides upstream of the nanH ORF. Northern blot analysis using an internal nanH probe revealed a single mRNA species with a length of 1.8 kb that could encode just the neuraminidase.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
203
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pubmed:geneSymbol |
nanH
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
914-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8093075-Amino Acid Sequence,
pubmed-meshheading:8093075-Bacteroides fragilis,
pubmed-meshheading:8093075-Base Sequence,
pubmed-meshheading:8093075-Blotting, Northern,
pubmed-meshheading:8093075-Consensus Sequence,
pubmed-meshheading:8093075-Molecular Sequence Data,
pubmed-meshheading:8093075-Molecular Weight,
pubmed-meshheading:8093075-Mutagenesis, Site-Directed,
pubmed-meshheading:8093075-Neuraminidase,
pubmed-meshheading:8093075-Open Reading Frames,
pubmed-meshheading:8093075-RNA, Messenger
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pubmed:year |
1994
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pubmed:articleTitle |
Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene.
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pubmed:affiliation |
Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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