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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-10-18
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pubmed:abstractText |
The 5'-TAR region of HIV-1 mRNA is highly conserved amongst different HIV-1 isolates. We thus investigated the potential for in vivo targeting of the TAR RNA element by a hammerhead ribozyme. The use of the CAT reporter gene linked to the HIV1-LTR, in transient assays, reveals that a hammerhead ribozyme directed towards the first GUC of HIV-1 mRNA can efficiently inhibit CAT protein expression. We show that this inhibition is sequence-specific and probably due to a cleavage activity rather than an antisense effect. We show also that a hammerhead ribozyme that is inactive in vitro is capable of inhibiting CAT protein expression in a cellular environment. These results suggest that the targeting of the HIV-1 LTR by a hammerhead ribozyme constitutes a viable approach for anti-HIV therapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
203
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
889-98
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8093072-Base Sequence,
pubmed-meshheading:8093072-Cell Line,
pubmed-meshheading:8093072-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8093072-Gene Expression,
pubmed-meshheading:8093072-Gene Products, tat,
pubmed-meshheading:8093072-Genes, Reporter,
pubmed-meshheading:8093072-HIV Long Terminal Repeat,
pubmed-meshheading:8093072-HIV-1,
pubmed-meshheading:8093072-Molecular Sequence Data,
pubmed-meshheading:8093072-Plasmids,
pubmed-meshheading:8093072-RNA, Catalytic,
pubmed-meshheading:8093072-RNA, Messenger,
pubmed-meshheading:8093072-Transfection,
pubmed-meshheading:8093072-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1994
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pubmed:articleTitle |
Ribozyme targeting of HIV-1 LTR.
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pubmed:affiliation |
ICGM, INSERM U 363, Université Paris V, Hospital Cochin, Paris, France.
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pubmed:publicationType |
Journal Article
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