Linked Life Data

8092488

Source:http://linkedlifedata.com/resource/pubmed/id/8092488

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1994-10-20
pubmed:abstractText
The goal of this work was to develop a generic approach for producing affinity chromatographic columns which can be regenerated. Concanavalin A (Con A) was immobilized adsorptively by an in situ method onto a zirconium dioxide (zirconia) chromatographic support and used to resolve chromophorically labeled monosaccharides. The Con A was then removed from the zirconia by flushing with base. The same column was regenerated by applying a fresh aliquot of Con A. This cycle was repeated several times to demonstrate consistency in the loading capacity and the stability of the underlying zirconia support. Finally we used glutaraldehyde to cross-link the Con A to increase the long-term stability of the column. Hydrolyzing the protein with acid allowed it to be removed under alkaline conditions and the column regenerated simply by adding more Con A followed by glutaraldehyde cross-linking.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2584-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Easily regenerable affinity chromatographic zirconia-based support with concanavalin A as a model ligand.
pubmed:affiliation
Department of Chemistry, University of Minnesota, Minneapolis 55455-0431.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't