Linked Life Data

8089482

Source:http://linkedlifedata.com/resource/pubmed/id/8089482

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-10-14
pubmed:abstractText
Ig isotype switching in B lymphocytes is preceded by transcription of the corresponding unrearranged, or germ-line (GL), CH gene. The promoter of mouse GL C gamma 1 transcripts has been shown to be located within a 349-bp KpnI/Bg/II fragment, extending from -147 to +202 bp relative to the first transcription initiation site. By the electrophoretic mobility shift assay, we have analyzed nuclear extracts from three B cell lines and splenic B cells for the presence of proteins binding to this fragment. We show that they give different patterns of DNA binding, implying significant complexity in the regulation of this locus. We focused on the sIgM+ mouse B lymphoma line L10A6.2 that has been shown able to confer responsiveness of the GL gamma 1 promoter to phorbol ester plus IL-4. Activation of this cell line results in altered expression of several nuclear DNA-binding complexes involving two members of the C/enhancer-binding protein (EBP) family of transcription factors, namely C/EBP beta (nuclear factor (NF)-IL-6/LAP) and Ig/EBP-1 (C/EBP gamma). The complexes bind to two C/EBP elements, one at about -115 bp and one near the first RNA start site. In normal B cells stimulated by LPS or IL-4, new complexes appear that bind to C/EBP and NF-IL-4 elements, respectively, located within the -125/-101 region. The -125/-101 segment previously has been shown to be required for transcriptional activation. We discuss these findings in relation to the presence of consensus C/EBP binding sites in other IL-4-regulated promoters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2983-95
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Activation of the Ig germ-line gamma 1 promoter. Involvement of C/enhancer-binding protein transcription factors and their possible interaction with an NF-IL-4 site.
pubmed:affiliation
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't