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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
2
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pubmed:dateCreated |
1994-10-20
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pubmed:abstractText |
Calcyclin is a member of the S100 family of proteins, many of which are encoded by genes that have been localized to the proximal long arm of human chromosome 1 (bands q21-q22). A 450-kb yeast artificial chromosome clone containing the human calcyclin gene was identified by PCR screening and used as a probe for fluorescence in situ hybridization (FISH). Along with the expected hybridization to 1q21, simultaneous, specific hybridization to the centromeric region of the short arm of chromosome 1 was also observed. An identical pattern of hybridization was observed when microdissected 1q21 DNA sequences were used as a probe for FISH, confirming the presence of homologous sequences flanking both sides of the centromere of human chromosome 1. These results are consistent with a model in which human chromosome 1 arose by insertion of the centromere and heterochromatin into an ancestral chromosome containing chromosome-specific repetitive sequences.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
May
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|
pubmed:issn |
0888-7543
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
15
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|
pubmed:volume |
21
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|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
359-63
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8088830-Animals,
pubmed-meshheading:8088830-Base Sequence,
pubmed-meshheading:8088830-Calcium-Binding Proteins,
pubmed-meshheading:8088830-Cell Cycle Proteins,
pubmed-meshheading:8088830-Centromere,
pubmed-meshheading:8088830-Chromosome Mapping,
pubmed-meshheading:8088830-Chromosomes, Artificial, Yeast,
pubmed-meshheading:8088830-Chromosomes, Human, Pair 1,
pubmed-meshheading:8088830-DNA Primers,
pubmed-meshheading:8088830-Epidermal Growth Factor,
pubmed-meshheading:8088830-Humans,
pubmed-meshheading:8088830-In Situ Hybridization, Fluorescence,
pubmed-meshheading:8088830-Mice,
pubmed-meshheading:8088830-Molecular Sequence Data,
pubmed-meshheading:8088830-Polymerase Chain Reaction,
pubmed-meshheading:8088830-S100 Proteins,
pubmed-meshheading:8088830-Sequence Homology, Nucleic Acid
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|
pubmed:year |
1994
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|
pubmed:articleTitle |
Direct evidence for homologous sequences on the paracentric regions of human chromosome 1.
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pubmed:affiliation |
Department of Dermatology, University of Michigan, Ann Arbor 48109-0672.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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