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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-10-20
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pubmed:abstractText |
The SUP35 gene of yeast Saccharomyces cerevisiae encodes a 76.5-kD ribosome-associated protein (Sup35p), the C-terminal part of which exhibits a high degree of similarity to EF-1 alpha elongation factor, while its N-terminal region is unique. Mutations in or overexpression of the SUP35 gene can generate an omnipotent suppressor effect. In the present study the SUP35 wild-type gene was replaced with deletion alleles generated in vitro that encode Sup35p lacking all or a part of the unique N-terminal region. These 5'-deletion alleles lead, in a haploid strain, simultaneously to an antisuppressor effect and to loss of the non-Mendelian determinant [psi+]. The antisuppressor effect is dominant while the elimination of the [psi+] determinant is a recessive trait. A set of the plasmid-borne deletion alleles of the SUP35 gene was tested for the ability to maintain [psi+]. It was shown that the first 114 amino acids of Sup35p are sufficient to maintain the [psi+] determinant. We propose that the Sup35p serves as a trans-acting factor required for the maintenance of [psi+].
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-1523883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-17246215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-1874413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-2050148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-2080663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-2511002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-2841115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3047009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3059716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-321935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3280807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3319781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3323850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-341157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-3522920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-372549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-395029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-4583607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-5485912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-6336730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-6757053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-6795357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-6987083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-7002720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-7031258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-775294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-785283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8088512-8469113
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:geneSymbol |
PNM2,
SUP35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
671-6
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:8088512-Amino Acid Sequence,
pubmed-meshheading:8088512-Extrachromosomal Inheritance,
pubmed-meshheading:8088512-Fungal Proteins,
pubmed-meshheading:8088512-Gene Deletion,
pubmed-meshheading:8088512-Gene Expression Regulation, Fungal,
pubmed-meshheading:8088512-Genes, Dominant,
pubmed-meshheading:8088512-Genes, Fungal,
pubmed-meshheading:8088512-Genes, Suppressor,
pubmed-meshheading:8088512-Peptide Elongation Factors,
pubmed-meshheading:8088512-Peptide Termination Factors,
pubmed-meshheading:8088512-Plasmids,
pubmed-meshheading:8088512-Prions,
pubmed-meshheading:8088512-Protein Biosynthesis,
pubmed-meshheading:8088512-Saccharomyces cerevisiae,
pubmed-meshheading:8088512-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8088512-Structure-Activity Relationship,
pubmed-meshheading:8088512-Suppression, Genetic,
pubmed-meshheading:8088512-Trans-Activators
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pubmed:year |
1994
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pubmed:articleTitle |
The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae.
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pubmed:affiliation |
Institute of Experimental Cardiology, Cardiology Research Center, Moscow, Russia.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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