Linked Life Data

8087864

Source:http://linkedlifedata.com/resource/pubmed/id/8087864

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-20
pubmed:abstractText
Recently, we have shown that T cells exposed to concentrations of prostaglandin E2 (PGE2) or the beta-adrenergic receptor agonist isoproterenol (ISO) that elicit equimolar levels of cAMP accumulation do not inhibit anti-CD3 monoclonal antibody-induced T cell proliferation to the same extent. This report extends these studies by investigating the induction of cAMP-dependent protein kinase (PKA) in T cells stimulated with PGE2 or ISO. The kinetics of PKA activity induced by PGE2 or ISO in T cells are similar but PGE2 induces more PKA activity. When T cells were treated with concentrations of PGE2 or ISO that elicited similar PKA activities, PGE2 was found to be more immunosuppressive than ISO. T cells stimulated with PGE2 or ISO showed similar levels of increased PKA activity in both the cytosolic and the particulate fractions. Quantitation of the activity of PKA I and PKA II isozymes in T cells stimulated with PGE2 or ISO revealed that both types were activated; however, while PGE2 induced the utilization of an equal amount of both isozymes in T cells, ISO-treated cells utilized twice as much PKA I compared to PKA II. Overall, these results suggest that qualitative differences in the concentration of cAMP and PKA activity are important elements in modulatory T cell proliferative responses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
182-94
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Induction of cAMP-dependent protein kinase (PKA) activity in T cells after stimulation of the prostaglandin E2 or the beta-adrenergic receptors: relationship between PKA activity and inhibition of anti-CD3 monoclonal antibody-induced T cell proliferation.
pubmed:affiliation
Department of Microbiology and Immunology, University of Kentucky Medical Center, Lexington 40536-0084.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.