Linked Life Data

8087428

Source:http://linkedlifedata.com/resource/pubmed/id/8087428

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-10-18
pubmed:abstractText
The biotransformation of the Fusarium mycotoxins deoxynivalenol and zearalenone by the normal bacterial gut flora of pigs was examined in this in vitro study. For that purpose, suspensions of intestinal contents (duodenum, jejunum, caecum, colon, rectum) of porcine origin were incubated anaerobically with deoxynivalenol (DON) or zearalenone (ZEA). DON and ZEA were degraded by the flora of the caudal segments (caecum, colon, rectum) of the gut--particularly the colon content--whereas the microorganisms of the cranial segments (duodenum, jejunum) exhibited no transforming activity. DON was showed to be deepoxidated, ZEA was hydrolyzed to alpha-zearalenol and an unknown metabolite. The transformation of DON was correlated with a loss of cytotoxicity, which could be demonstrated in the MTT(3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium++ + bromide)-cell-culture assay using swine kidney cells as target cells. The results of the study presented here correspond with the data found in in vivo studies. On the basis of these findings one could conclude that this in vitro method seems to be well suited to the study of the transformation of mycotoxins by the microflora of the gut. The in vitro study is cheaper than a feeding trial, and the preliminary information on the metabolism of mycotoxins obtained in such studies is helpful in designing feeding trials more clearly. Besides the simple and fast handling, reproducibility and the protection of the animals studied are further advantages of this in vitro method. In connection with the MTT-cell-culture assay, additional information about the cytotoxic potential of the bacterial transformation products can be obtained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1056-9014
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
105-10
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
In vitro transformation of the Fusarium mycotoxins deoxynivalenol and zearalenone by the normal gut microflora of pigs.
pubmed:affiliation
Institute for Medical Microbiology, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany.
pubmed:publicationType
Journal Article