Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
36
|
pubmed:dateCreated |
1994-10-18
|
pubmed:abstractText |
Oxygen-evolving photosystem II complexes were isolated from the green alga Chlamydomonas reinhardtii by selective solubilization of thylakoid membranes with dodecyl maltoside followed by density gradient centrifugation and anion-exchange chromatography. In the presence of CaCl2 and K3[Fe(CN)6] the complexes evolved oxygen at rates exceeding 1000 mumol (mg of chl)-1 h-1. The particles contained 40 chlorophylls a and had properties very similar to those of PSII isolated from higher plants. Chlamydomonas reinhardtii is now the first organism which can be used for both site-directed mutagenesis and detailed biochemical and biophysical characterization of oxygen-evolving photosystem II. It seems therefore to be an ideal model organism for investigation of structure-function relationships in photosynthetic oxygen evolution.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0006-2960
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
13
|
pubmed:volume |
33
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
10906-10
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:8086407-Animals,
pubmed-meshheading:8086407-Chlamydomonas reinhardtii,
pubmed-meshheading:8086407-Chromatography, Ion Exchange,
pubmed-meshheading:8086407-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8086407-Oxygen,
pubmed-meshheading:8086407-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:8086407-Photosystem II Protein Complex
|
pubmed:year |
1994
|
pubmed:articleTitle |
Purification and characterization of oxygen-evolving photosystem II core complexes from the green alga Chlamydomonas reinhardtii.
|
pubmed:affiliation |
Department of Membrane Biochemistry, Max Planck Institute for Biochemistry, Martinsried, Germany.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|