Linked Life Data

8082723

Source:http://linkedlifedata.com/resource/pubmed/id/8082723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-12
pubmed:abstractText
A cell cycle ts mutant, tsJT663, isolated from Fischer rat cell line 3Y1, was characterized. The execution point of tsJT663 was approximately 2 h before S phase, starting either from a G0 (quiescent) state or from the previous cell cycle. Progression of the cell cycle other than from G0/G1 to S phase was temperature insensitive. The restriction (R) point was also estimated as about 2 h before S phase. When tsJT663 cells at log phase were transferred to 40 degrees C, the cell number increased and then decreased within 2 days and thereafter the cells were growth arrested stably for more than 4 weeks. When the serum-stimulated cells were cultured at 34 degrees C for 6 h at 40 degrees C for various period of time and then at 34 degrees C up to the end of the experiments at 30 h, the frequency of cells entering S phase declined depending upon the duration of culture at 40 degrees C. Assuming that the decline of the frequency of tsJT663 cells capable of entering S phase corresponded to the decline of a putative material required for cells to pass the R point toward S phase, the half-life of this material at 40 degrees C was 1 h. These results are consistent with a tentative conclusion that tsJT663 is a cell cycle ts mutant which does not prepare at nonpermissive temperature for passing the restriction point toward S phase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
214
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-14
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Isolation of a mammalian cell cycle ts mutant, tsJT663, defective in preparation for passing restriction point.
pubmed:affiliation
Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't