Linked Life Data

8081732

Source:http://linkedlifedata.com/resource/pubmed/id/8081732

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-10
pubmed:abstractText
A monoclonal antibody (mAb) 8B7, that recognizes specifically the alpha 1-subunit of dihydropyridine-sensitive calcium channels in skeletal muscle, exerted a dose-dependent positive chronotropic effect on cultured spontaneously beating neonatal rat cardiac myocytes. The antibody-induced increase in beating frequency was similar to that elicited by 1 microM of (-)-isoprenaline. The mAb 8B7 recognized a polypeptide that is analogous to the 170-kDa calcium channel alpha 1-subunit of rabbit skeletal muscle in wheat germ lectin purified proteins solubilized from cultured rat heart myocytes by Western blot analysis. Furthermore, the mAb 7C3, previously shown to recognize specifically the skeletal muscle calcium channel beta-subunit, reacted with a 55-kDa polypeptide in these cultured myocytes. No immunoreactivity, however, was obtained when proteins solubilized from both neonatal or adult rat heart tissue were employed in Western blotting or immunoprecipitation experiments. RNA-hybridization analysis revealed that primary cultures derived from neonatal rat hearts expressed two types of mRNA encoding the skeletal muscle as well as the cardiac calcium channel alpha 1-subunit, while neonatal rat heart tissue expressed only the message for cardiac calcium channel. These results provide evidence for a functional expression of skeletal muscle-type calcium channel subunits during the cultivation of neonatal rat heart cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1060-6823
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-52
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Detection of skeletal muscle calcium channel subunits in cultured neonatal rat cardiac myocytes.
pubmed:affiliation
Max-Delbrück Centrum für molekulare Medizin, Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't