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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1994-10-5
|
| pubmed:abstractText |
Ig receptor (IgR) on the surface of B cells mediates the Ag-specific stimulatory signal for B cell proliferation and differentiation. In immature B cells, the stimulatory signal causes an inhibitory effect which is believed to be a key phenomenon in B cell tolerance or B cell anergy. Here, we studied the molecular mechanism of the inhibitory response of the IgR-mediated signal transduction that results in the programmed cell death of immature B cells. To analyze the downstream molecules of the IgR-mediated signal transduction, we prepared a mAb against a 160-kDa membrane protein (p160) that can coprecipitate the kinase molecule(s) acting on serine, threonine, and tyrosine residues. Anti-IgR stimulation induces the increase of the kinase activity coprecipitated with the p160 protein in mature B cell BAL17 and normal adult spleen B cells. This result suggest that the p160-associated kinase activity is one of the downstream events of the IgR-mediated signal transduction cascade. Interestingly, immature B cell lymphoma WEHI-231 and the neonatal spleen B cells showed the adverse reaction of the p160-associated kinase which results in the transient loss of the kinase activity. Moreover, the transient decrease of the p160-associated kinase was caused by the tyrosine phosphatase activity induced by the stimulation of IgR in WEHI-231. The results suggest that this molecular difference in the downstream events of the IgR-mediated signal transduction between immature B cells and mature B cells already begins at the transmembrane level in the IgR-mediated signal transduction pathway.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
153
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2381-93
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:8077655-Animals,
pubmed-meshheading:8077655-Antibodies, Monoclonal,
pubmed-meshheading:8077655-Antigen-Antibody Complex,
pubmed-meshheading:8077655-B-Lymphocyte Subsets,
pubmed-meshheading:8077655-Blotting, Western,
pubmed-meshheading:8077655-Calcium,
pubmed-meshheading:8077655-Cell Line,
pubmed-meshheading:8077655-Membrane Proteins,
pubmed-meshheading:8077655-Mice,
pubmed-meshheading:8077655-Mice, Nude,
pubmed-meshheading:8077655-Molecular Weight,
pubmed-meshheading:8077655-Precipitin Tests,
pubmed-meshheading:8077655-Protein Tyrosine Phosphatases,
pubmed-meshheading:8077655-Protein-Tyrosine Kinases,
pubmed-meshheading:8077655-Rats,
pubmed-meshheading:8077655-Rats, Wistar,
pubmed-meshheading:8077655-Receptors, Antigen, B-Cell,
pubmed-meshheading:8077655-Signal Transduction,
pubmed-meshheading:8077655-Spleen
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
B cell Ag receptor mediates different types of signals in the protein kinase activity between immature B cell and mature B cell.
|
| pubmed:affiliation |
Department of Immunology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|