Linked Life Data

8076836

Source:http://linkedlifedata.com/resource/pubmed/id/8076836

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-10-4
pubmed:abstractText
The polymerase chain reaction (PCR) was used to amplify individual exons of the gene (CNTF) coding for human ciliary neurotrophic factor (CNTF) directly from genomic DNA. Inclusion of deoxyuracil in place of thymine in the PCR primers permits removal of dU residues in the primer after amplification using uracil DNA glycosylase, generating single-stranded 3' overhangs. Thus, the individual exons were assembled to generate the full-length CNTF sequence. A similar strategy was also used to generate a chimeric gene (BDNF) encoding brain-derived neurotrophic factor (BDNF) with the pre-pro sequence of nerve growth factor (NGF). The method described allows direct amplification of coding sequence from genomic DNA and ordered assembly of amplified exons to generate a clone containing the complete coding sequence of the gene without the need for splicing; a clone which is equivalent to a cDNA clone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
146
pubmed:geneSymbol
CNTF
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-8
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Assembly and cloning of coding sequences for neurotrophic factors directly from genomic DNA using polymerase chain reaction and uracil DNA glycosylase.
pubmed:affiliation
Department of Cellular Biochemistry, Life Technologies, Inc., BRL, Gaithersburg, MD 20884-9980.
pubmed:publicationType
Journal Article