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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1975-11-8
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pubmed:abstractText |
BETA-Lactamases (EC. 3.5.2.6) from strains of Gram-negative bacteria have been studied using analytical isoelectric focusing. This permits a visual comparison of the patterns of beta-lactamase bands produced by enzymes from different organisms. Purification of crude intracellular preparations is unnecessary and the technique is sufficiently sensitive to demonstrate beta-lactamase in mutants previously reported to lack the enzyme. R that have not been distinguished from one another biochemically or immunologically can be differentiated by isoelectric focusing. Conversely, the enzymes specified by the R factors RTEM, R1 and RGN14, with identical isoelectric focusing patterns have the same biochemical properties. Chromosomal and R-factor-mediated beta-lactamases from single strains have been separated and their identities confirmed by immunoisoelectric focusing. R factor-mediated enzymes gave identical isoelectric focusing patterns irrespective of the host strain. Isoelectric focusing can therefore be used to observe the transfer of beta-lactamases carried by R factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-1287
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-78
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pubmed:dateRevised |
2009-10-27
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pubmed:meshHeading |
pubmed-meshheading:807678-Amidohydrolases,
pubmed-meshheading:807678-Anti-Bacterial Agents,
pubmed-meshheading:807678-Bacteria,
pubmed-meshheading:807678-Cell-Free System,
pubmed-meshheading:807678-Cephalosporinase,
pubmed-meshheading:807678-Conjugation, Genetic,
pubmed-meshheading:807678-Drug Resistance, Microbial,
pubmed-meshheading:807678-Enterobacteriaceae,
pubmed-meshheading:807678-Escherichia coli,
pubmed-meshheading:807678-Isoelectric Focusing,
pubmed-meshheading:807678-Klebsiella,
pubmed-meshheading:807678-Mutation,
pubmed-meshheading:807678-Penicillinase,
pubmed-meshheading:807678-Proteus,
pubmed-meshheading:807678-Proteus mirabilis,
pubmed-meshheading:807678-Pseudomonas aeruginosa,
pubmed-meshheading:807678-Species Specificity,
pubmed-meshheading:807678-beta-Lactams
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pubmed:year |
1975
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pubmed:articleTitle |
The use of analytical isoelectric focusing for detection and identification of beta-lactamases.
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pubmed:publicationType |
Journal Article
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