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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1994-10-3
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have isolated and characterized Xenopus cDNA clones for a new transcription factor that represents an early marker for the developing heart. The cDNAs encode a protein that we have designated GATA-4; it contains the highly conserved DNA-binding domain that characterizes this family of cell-type restricted transcriptional activators. Whole-embryo in situ analysis of Xenopus embryos demonstrates that the GATA-4 gene is transcribed in presumptive cardiac ventral mesoderm at the time that bilateral progenitors fuse and form the cardiac tube. GATA-4 is therefore the earliest molecular marker of cardiogenesis yet characterized. By stage 30, the GATA-4 mRNA is expressed in the developing atria and ventricles; at stage 38, cross-sections reveal that the gene is active in the endocardial layer, but not in myocardium. By stage 40, GATA-4 message is detected in the great vessels. In the adult frog, the GATA-4 gene is highly transcribed in heart and gut; lower levels of message are detected in various endoderm-derived tissues and gonads. Expression in the stomach is largely confined to the epithelium. The GATA-4 gene is first activated at stage 11; mRNA is initially present throughout the marginal zone of explants and later partially localized to the ventral marginal zone. GATA-4 mRNA is also detected at high levels in cultured endodermal explants derived from the vegetal region of early embryos. In mesoderm induction experiments, GATA-4 transcription is not induced in animal caps treated with activin or bFGF. The GATA-4 gene may provide a new early marker for studying the inductive processes that lead to the formation of the cardiovascular system and for the specification of the endocardial lineage.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0950-1991
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
118
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
817-27
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8076520-Amino Acid Sequence,
pubmed-meshheading:8076520-Animals,
pubmed-meshheading:8076520-Base Sequence,
pubmed-meshheading:8076520-Biological Markers,
pubmed-meshheading:8076520-DNA-Binding Proteins,
pubmed-meshheading:8076520-Endocardium,
pubmed-meshheading:8076520-GATA4 Transcription Factor,
pubmed-meshheading:8076520-Gene Expression,
pubmed-meshheading:8076520-Heart,
pubmed-meshheading:8076520-Molecular Sequence Data,
pubmed-meshheading:8076520-Multigene Family,
pubmed-meshheading:8076520-Muscles,
pubmed-meshheading:8076520-Organ Specificity,
pubmed-meshheading:8076520-Sequence Alignment,
pubmed-meshheading:8076520-Sequence Homology, Amino Acid,
pubmed-meshheading:8076520-Skin,
pubmed-meshheading:8076520-Transcription, Genetic,
pubmed-meshheading:8076520-Transcription Factors,
pubmed-meshheading:8076520-Viscera,
pubmed-meshheading:8076520-Xenopus,
pubmed-meshheading:8076520-Xenopus Proteins
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
GATA-4 is a novel transcription factor expressed in endocardium of the developing heart.
|
| pubmed:affiliation |
Children's Hospital, Harvard Medical School, Boston, MA 02115.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|