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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1975-11-6
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pubmed:abstractText |
The transformation of Bacillus subtilis by homologous deoxyribonucleic acid (DNA) made available by gently lysing a stable L-form or protoplast suspension was 3 to 10-fold more efficient than DNA isolated by conventional procedures. This increased transformation was not influenced by digestion with pronase, trypsin, or ribonuclease. Preincubation of isolated DNA with L-form lysates did not increase the transformation efficiency above that achieved with untreated, isolated DNA. In addition to displaying a higher efficiency of transformation, the DNA found in these gently prepared lysates was also able to co-transform heretofore unlinked markers at frequencies in excess of those found by congression. Comparison of the frequency of multiple marker transformations to single marker events as a function of DNA dilution conclusively proves that these markers originated from the same continuous strand of DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-13293190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-13728419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-13900279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-14255692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-14273661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-14293004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-16590310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4110922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4196254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4196974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4199220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4200839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4203903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4504368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4622631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4622632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4622900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4626501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4631599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4956757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4974083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4977972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4988045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-4992373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-5003662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-5004186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807561-5685998
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
987-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:807561-Bacillus subtilis,
pubmed-meshheading:807561-Bacteriolysis,
pubmed-meshheading:807561-DNA, Bacterial,
pubmed-meshheading:807561-Deoxyribonucleases,
pubmed-meshheading:807561-Genes,
pubmed-meshheading:807561-L Forms,
pubmed-meshheading:807561-Mutation,
pubmed-meshheading:807561-Pronase,
pubmed-meshheading:807561-Protoplasts,
pubmed-meshheading:807561-Ribonucleases,
pubmed-meshheading:807561-Thymidine,
pubmed-meshheading:807561-Transformation, Genetic,
pubmed-meshheading:807561-Tritium,
pubmed-meshheading:807561-Trypsin
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pubmed:year |
1975
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pubmed:articleTitle |
Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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