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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1994-9-29
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pubmed:abstractText |
To investigate the mechanisms of Na+ loading during metabolic inhibition (MI), [Na+]i and pHi were measured in quiescent guinea pig myocytes using fluorescent probes, sodium-binding benzofuran isophthalate and 2,7,bis(carboxyethyl)-5,6-carboxyfluorescein. When myocytes were exposed to MI (3.3 mM amobarbital and 5 microM carbonyl cyanide m-chlorophenylhydrazone, without glucose) for 20 min, [Na+]i increased from 8.3 +/- 0.7 mM to 17.7 +/- 1.3 mM (p < 0.01) and pHi decreased from 7.22 +/- 0.03 to 7.00 +/- 0.04 (p < 0.05). The inhibition of Na(+)-H+ exchange by hexamethylene amiloride (HMA) significantly attenuated the increase in [Na+]i during MI (9.3 +/- 0.9 mM; p < 0.01 vs MI without HMA). When a K(+)-free solution was perfused to inhibit the Na+/K+ pump in the presence of HMA, there was an immediate increase in [Na+]i during MI. Perfusion of a K(+)-free solution after 10 min of MI caused no change in the rate of the increase in [Na+]i. We concluded that 1) Na+/H+ exchange was an important mechanism for Na+ elevation during MI, and 2) the Na+/K+ pump was functional during the early phase of MI, but was inhibited 10 min after MI in this model.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-(N,N-hexamethylene)amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
203
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8074732-Amiloride,
pubmed-meshheading:8074732-Analysis of Variance,
pubmed-meshheading:8074732-Animals,
pubmed-meshheading:8074732-Cells, Cultured,
pubmed-meshheading:8074732-Female,
pubmed-meshheading:8074732-Fluorescent Dyes,
pubmed-meshheading:8074732-Guinea Pigs,
pubmed-meshheading:8074732-Homeostasis,
pubmed-meshheading:8074732-Hydrogen-Ion Concentration,
pubmed-meshheading:8074732-Kinetics,
pubmed-meshheading:8074732-Muscles,
pubmed-meshheading:8074732-Sodium,
pubmed-meshheading:8074732-Sodium-Hydrogen Antiporter,
pubmed-meshheading:8074732-Sodium-Potassium-Exchanging ATPase
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pubmed:year |
1994
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pubmed:articleTitle |
The role of Na+/H+ exchange and the Na+/K+ pump in the regulation of [Na+]i during metabolic inhibition in guinea pig myocytes.
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pubmed:affiliation |
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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