|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1994-9-26
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|
pubmed:abstractText |
A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A virus. The strain MB-7 induced plaque formation in FRhK-4 under agar overlay after 10-12 days of incubation. The PCR products of gene VP1 were cloned in E. coli and its primary structure was determined. MB-7 was shown to have more homology with HAV strains isolated in the USA and China.
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pubmed:language |
rus
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0507-4088
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
38
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
101-5
|
|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
pubmed-meshheading:8073747-Antigens, Viral,
pubmed-meshheading:8073747-Base Sequence,
pubmed-meshheading:8073747-Cloning, Molecular,
pubmed-meshheading:8073747-Cytopathogenic Effect, Viral,
pubmed-meshheading:8073747-DNA, Complementary,
pubmed-meshheading:8073747-DNA, Viral,
pubmed-meshheading:8073747-Hepatitis A,
pubmed-meshheading:8073747-Hepatovirus,
pubmed-meshheading:8073747-Humans,
pubmed-meshheading:8073747-Microscopy, Electron,
pubmed-meshheading:8073747-Molecular Sequence Data,
pubmed-meshheading:8073747-Neutralization Tests,
pubmed-meshheading:8073747-Siberia,
pubmed-meshheading:8073747-Virus Cultivation
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|
pubmed:articleTitle |
[The isolation and study of the characteristics of a cytopathic strain of the hepatitis A virus].
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|
pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
|
