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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-9-29
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pubmed:databankReference |
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pubmed:abstractText |
Mutational analysis identified a C-terminal region of 78 amino acids within the cytoplasmic domain of the human 75 kDa tumor necrosis factor receptor (TNF-R2) that is required for signal transduction. This region was subsequently shown to mediate the interaction of cytoplasmic factors with TNF-R2. Two of these factors were isolated and molecularly cloned using biochemical purification and the yeast two-hybrid system. TNF receptor-associated factor 1 (TRAF1) and TRAF2 are the first two members of a novel protein family containing a novel C-terminal homology region, the TRAF domain. In addition, TRAF2 contains an N-terminal RING finger motif. TRAF1 and TRAF2 can form homo- and heterotypic dimers. Our analysis indicates that TRAF1 and TRAF2 are associated with the cytoplasmic domain of TNF-R2 in a heterodimeric complex in which TRAF2 contacts the receptor directly. TRAF1 interacts with TNF-R2 indirectly through heterodimer formation with TRAF2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
681-92
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:8069916-Amino Acid Sequence,
pubmed-meshheading:8069916-Animals,
pubmed-meshheading:8069916-Antigens, CD,
pubmed-meshheading:8069916-Base Sequence,
pubmed-meshheading:8069916-Cloning, Molecular,
pubmed-meshheading:8069916-Cytoplasm,
pubmed-meshheading:8069916-Humans,
pubmed-meshheading:8069916-Mice,
pubmed-meshheading:8069916-Models, Biological,
pubmed-meshheading:8069916-Molecular Sequence Data,
pubmed-meshheading:8069916-Organ Specificity,
pubmed-meshheading:8069916-Protein Conformation,
pubmed-meshheading:8069916-Protein Structure, Tertiary,
pubmed-meshheading:8069916-Proteins,
pubmed-meshheading:8069916-RNA, Messenger,
pubmed-meshheading:8069916-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:8069916-Receptors, Tumor Necrosis Factor, Type II,
pubmed-meshheading:8069916-Recombinant Fusion Proteins,
pubmed-meshheading:8069916-Sequence Alignment,
pubmed-meshheading:8069916-Sequence Analysis,
pubmed-meshheading:8069916-Sequence Analysis, DNA,
pubmed-meshheading:8069916-Sequence Homology, Amino Acid,
pubmed-meshheading:8069916-Signal Transduction,
pubmed-meshheading:8069916-TNF Receptor-Associated Factor 1,
pubmed-meshheading:8069916-TNF Receptor-Associated Factor 2
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pubmed:year |
1994
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pubmed:articleTitle |
A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor.
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pubmed:affiliation |
Department of Molecular Biology, Genentech, Incorporated, South San Francisco, California 94080.
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pubmed:publicationType |
Journal Article
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