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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-9-23
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pubmed:abstractText |
Information on the transmembrane signaling events and subsequent biochemical processes initiated by ciliary neurotrophic factor (CNTF) receptor activation in neurons is lacking. SH-SY5Y cells, a human neuroblastoma cell line expressing CNTF receptors, were used to study metabolic changes associated with functional ligand-receptor interactions. Real-time measurements quantifying the rate of extracellular acidification by SH-SY5Y cells (a measure of metabolic activity) were made using a silicon-based cytosensor. Application of recombinant human CNTF (rhCNTF) to resting SH-SY5Y cells increased their acidification rate in a concentration and time-dependent manner with an apparent EC50 of 60 ng/ml. Pretreatment of cells with phosphatidylinositol-specific phospholipase C (PI-PLC) prevented the CNTF, but not an NGF-stimulated increase in acidification rate. Collectively, these results demonstrate that: (1) SH-SY5Y cells express functional CNTF receptors; and (2) the initial signal transduction mechanism activated by the CNTF receptor in SH-SY5Y cells is distinct from that activated by the NGF receptor; however, both may ultimately stimulate the same downstream biochemical messengers to increase cellular metabolism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ciliary Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoinositide Phospholipase C,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Diester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Ciliary Neurotrophic...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-8993
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
646
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
327-31
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8069684-Animals,
pubmed-meshheading:8069684-Cell Line,
pubmed-meshheading:8069684-Ciliary Neurotrophic Factor,
pubmed-meshheading:8069684-Dose-Response Relationship, Drug,
pubmed-meshheading:8069684-Humans,
pubmed-meshheading:8069684-Hydrogen-Ion Concentration,
pubmed-meshheading:8069684-Kinetics,
pubmed-meshheading:8069684-Mice,
pubmed-meshheading:8069684-Nerve Growth Factors,
pubmed-meshheading:8069684-Nerve Tissue Proteins,
pubmed-meshheading:8069684-Neuroblastoma,
pubmed-meshheading:8069684-Phosphatidylinositol Diacylglycerol-Lyase,
pubmed-meshheading:8069684-Phosphoinositide Phospholipase C,
pubmed-meshheading:8069684-Phosphoric Diester Hydrolases,
pubmed-meshheading:8069684-Receptor, Ciliary Neurotrophic Factor,
pubmed-meshheading:8069684-Receptors, Growth Factor,
pubmed-meshheading:8069684-Receptors, Nerve Growth Factor,
pubmed-meshheading:8069684-Recombinant Proteins,
pubmed-meshheading:8069684-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Recombinant human ciliary neurotrophic factor stimulates the metabolic activity of SH-SY5Y cells as measured by a cytosensor microphysiometer.
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pubmed:affiliation |
Department of Neurosciences, Syntex Discovery Research, Palo Alto, CA 94304.
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pubmed:publicationType |
Journal Article
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